General Notes
This product (Catalog No. DPABH-13467) is a rabbit-derived polyclonal antibody raised against human UCP3. This antibody exhibits cross-reactivity with UCP3 from mouse and rat. It has been affinity purified by Protein A. Uncoupling proteins (UCPs) are a family of inner mitochondrial membrane proteins that facilitate the leakage of protons across the membrane, thereby uncoupling the electron transport chain from ATP synthesis and dissipating the proton gradient as heat rather than chemical energy. Discovered in the 1970s, UCPs were first identified in brown adipose tissue, where they enable adaptive thermoregulation in response to cold exposure. The prototypical member, UCP1, is predominantly expressed in brown adipose tissue and is activated by free fatty acids and inhibited by purine nucleotides like GDP, driving heat production critical for maintaining body temperature in mammals, particularly newborns and hibernating animals. In contrast, UCP2 and UCP3, which share high sequence homology with UCP1, are more widely distributed—UCP2 in white adipose tissue, liver, pancreas, and immune cells, and UCP3 primarily in skeletal muscle—and exhibit milder uncoupling activity primarily linked to ROS mitigation and fatty acid metabolism rather than substantial thermogenesis. These proteins reduce superoxide generation by mildly depolarizing the mitochondrial membrane, protecting cells from oxidative damage during high metabolic states. Additional homologues, such as brain-specific UCP4 and UCP5, further extend this family's influence to neuroprotection, potentially mitigating conditions like stroke and neurodegeneration by similar mechanisms.
DPABH-13467 is suitable for use in WB 1 (500-1:2000), IHC 1 (50-1:200) applications.
DPABH-13467 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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