Anti-Human TRPM2 Polyclonal Antibody (DPABH-27352)

Rabbit Anti-Human TRPM2 Polyclonal Antibody for WB, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Immunogen range: Between 650 and 700
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
Western Blot: 1:2,000 - 1:10,000
Immunoprecipitation: 2 - 10 µg/mg lysate
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Detection of human TRPM2 by western blot of immunoprecipitates.
Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells.
Antibodies: Affinity purified antibody used for IP at 6 µg per reaction.
Detection Chemiluminescence with an exposure time of 30 seconds.
Detection of human TRPM2 by western blot.
Samples: Jurkat Membrane Prep (50, 15, 5 µg).
Antibody: Affinity purified antibody used for WB at 0.1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.

Target


Alternative Names
TRPM2; transient receptor potential cation channel, subfamily M, member 2; TRPC7; transient receptor potential cation channel subfamily M member 2; EREG1; KNP3
Entrez Gene ID
UniProt ID

Citations


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References


Xu, SZ; Zeng, B; et al. Activation of TRPC Cationic Channels by Mercurial Compounds Confers the Cytotoxicity of Mercury Exposure. TOXICOLOGICAL SCIENCES 125:56-68(2012).
Hecquet, CM; Ahmmed, GU; et al. Role of TRPM2 channel in mediating H2O2-induced Ca2+ entry and endothelial hyperpermeability. CIRCULATION RESEARCH 102:347-355(2008).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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