Anti-Human TRPM2 Polyclonal Antibody (DPABH-27352)
Rabbit Anti-Human TRPM2 Polyclonal Antibody for WB, IP
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Immunogen range: Between 650 and 700
Western Blot: 1:2,000 - 1:10,000
Immunoprecipitation: 2 - 10 µg/mg lysate
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Detection of human TRPM2 by western blot of immunoprecipitates.
Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells.
Antibodies: Affinity purified antibody used for IP at 6 µg per reaction.
Detection Chemiluminescence with an exposure time of 30 seconds.
Detection of human TRPM2 by western blot.
Samples: Jurkat Membrane Prep (50, 15, 5 µg).
Antibody: Affinity purified antibody used for WB at 0.1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
TRPM2; transient receptor potential cation channel, subfamily M, member 2; TRPC7; transient receptor potential cation channel subfamily M member 2; EREG1; KNP3
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Naylor, J; Milligan, CJ; et al. Production of a specific extracellular inhibitor of TRPM3 channels. BRITISH JOURNAL OF PHARMACOLOGY 155:567-573(2008).
Tran, VT; Ho, PT; et al. Mechanotransduction Channels of the Trabecular Meshwork. CURRENT EYE RESEARCH 39:291-303(2014).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.