Rabbit Anti-TOP1 monoclonal antibody, clone TD70-14

Sign language uses manual-visual to convey meaning. The style is expressed through manual sign flow in combination with non-manual elements. Sign gestures interpreted in the meaning of words, letters, and numbers. This study proposed Two-stream Convolutional Neural Networks (CNN) to recognize and classify words in hand motion images of video form. Two-stream CNN works with two processes, namely spatial and temporal stream. Spatial flow detects edges and overall global features. While temporal flow identifies local action features in stacked optical flow images of 10 frames, each stream passed Softmax function. Average Fusion function combines both of streams. Two-stream separated training reduced computing time and overcome resource limitations. In building a CNN two-stream model, a specific configuration is needed to update the weight during training such as VGG - SGD, Resnet - Adam, Resnet - SGD, Xceptionnet - Adam, and Xceptionnet - SGD. The result gave the best precision used Xceptionnet SGD of spatial flow and Xceptionnet Adam of temporal flow configuration. The architecture gave precision 89.4% of a combination of one choice or Top1 is 89.4% and 99.4% of the five choices or Top5.

The SUMO-dependent ubiquitin ligase Slx8 plays key roles in promoting genome stability, including the processing of trapped Topoisomerase I (Top1) cleavage complexes and removal of toxic SUMO conjugates. We show that it is the latter function that constitutes Slx8's primary role in fission yeast. The SUMO conjugates in question are formed by the SUMO ligase Pli1, which is necessary for limiting spontaneous homologous recombination when Top1 is present. Surprisingly there is no requirement for Pli1 to limit recombination in the vicinity of a replication fork blocked at the programmed barrier RTS1. Notably, once committed to Pli1-mediated SUMOylation Slx8 becomes essential for genotoxin resistance, limiting both spontaneous and RTS1 induced recombination, and promoting normal chromosome segregation. We show that Slx8 removes Pli1-dependent Top1-SUMO conjugates and in doing so helps to constrain recombination at RTS1. Overall our data highlight how SUMOylation and SUMO-dependent ubiquitylation by the Pli1-Slx8 axis contribute in different ways to maintain genome stability.