General Notes
This product (Catalog No. DPABH-00790) is a rabbit-derived polyclonal antibody raised against human TF, targeting the amino acid region 1–251 of the protein. It has been affinity purified by Protein A. Tissue factor (TF), also known as coagulation factor III or thromboplastin, is a single-pass transmembrane glycoprotein that functions as the principal cellular initiator of blood coagulation in the extrinsic pathway. Encoded by the F3 gene on chromosome 1p21.3, TF is normally expressed on the surfaces of perivascular cells such as fibroblasts, smooth muscle cells, and pericytes, but not on circulating blood cells or endothelial cells under steady-state conditions, thereby preventing inappropriate clotting in the vasculature. Upon vascular injury, TF is exposed to circulating factor VII (FVII), which it binds with high affinity to form the TF-FVIIa complex; this complex allosterically activates FVII to FVIIa and catalyzes the activation of factors IX and X, leading to thrombin generation, fibrin clot formation, and platelet activation for hemostasis. TF's discovery dates back to the early 20th century when it was identified as "thromboplastin" by researchers like Morawitz and Loeb, with its molecular cloning achieved in 1981.
DPABH-00790 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-00790 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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