Immunogen
Recombinant human WWTR1,TAZ protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-400 aa of BC014052
General Notes
This product (Catalog No. DPABH-11954) is a rabbit-derived polyclonal antibody raised against human WWTR1, targeting the amino acid region 1–400 of the protein. This antibody exhibits cross-reactivity with Human WWTR1 from rat. It has been affinity purified by Protein A. TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.
DPABH-11954 is suitable for use in Western blot (WB) (1:1,000–1:8,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF/ICC (1:50–1:500), flow cytometry (FC) (0.25 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-11954 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
COA
Publication 
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