Anti-SUPT4H1 monoclonal antibody, clone FQS7211(3)

Antibody Isotype

IgG

Clone

FQS7211(3)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide corresponding to residues in Human Suppressor of Ty 4 homolog 1 (P63272).

Conjugate

Application Notes

WB: 1/1000 - 1/10000.

Alternative Names

SUPT4H1; suppressor of Ty 4 homolog 1 (S. cerevisiae); suppressor of Ty (S.cerevisiae) 4 homolog 1 , SUPT4H; transcription elongation factor SPT4; SPT4H; hSPT4; DSIF p14; DSIF small subunit; DRB sensitivity-inducing factor small subunit; DRB sensitivity-i

Entrez Gene ID

UniProt ID

Antigen Description

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3 terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Pathway

Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Disease, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem; Formation of RNA Pol II elongation complex, organism-specific biosystem; Formation of the Early Elongation Complex, organism-specific biosystem; Formation of the HIV-1 Early Elongation Complex, organism-specific biosystem;