Specifications
Species Reactivity
Rat; Human
Immunogen
Synthetic peptide corresponding to a C-terminal portion of native rat 5-alpha reductase type 2 conjugated to human thyroglobulin. The amino acid sequence is as follows:QAFYHHRFYLKMFKDYPKSRKAL
Target
Alternative Names
Srd5a2; 5-alpha reductase type 2; steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); S5AR 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2; 5 alpha SR2; SR type 2; Steroid 5 alpha r
Product Background
Antigen Description
enzyme which converts testosterone to dihydrotestosterone (DHT); also acts on progesterone and corticosterone; plays a central role in sexual differentiation and androgen physiology
Pathway
Prostate cancer, organism-specific biosystem; Prostate cancer, conserved biosystem; Steroid hormone biosynthesis, organism-specific biosystem; Steroid hormone biosynthesis, conserved biosystem.
Citations
Publication ()
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Prostate cancer cell growth characteristics in serum and prostate-conditioned media from moderate-intensity exercise-trained healthy and tumor-bearing rats
Alexander B Opoku-Acheampong, Dryden R Baumfalk, Andrew G Horn, Olivia N Kunkel, Charan K Ganta, Danielle J McCullough, Dietmar W Siemann, Judy Muller-Delp, Bradley J Behnke
Applications: IHC
Reactive species: Rat
"Abstract: Physical activity is associated with diminished risk of several cancers, and preclinical studies suggest exercise training may alter tumor cell growth in certain tissue(s) (e.g., adipose). From moderate-intensity exercise-trained rats versus sedentary controls, we hypothesized 1) there will be a decreased prostate cancer cell viability and migration in vitro and, within the prostate, a reduced 5α-reductase 2 (5αR2) and increased caspase-3 expression, and 2) that exercise training in tumor-bearing (TB) animals will demonstrate a reduced tumor cell viability in prostate-conditioned media. Serum and prostate were harvested from sedentary or exercise-trained (treadmill running, 10-11 weeks) immune-competent (Copenhagen; n = 20) and -deficient (Nude; n = 18) rats. AT-1 and PC-3 prostate cancer cells were grown in one or more of the following: serum-supplemented media (SSM), SSM from TB rats (SSM-TB), prostate-conditioned media (PCM) or PCM from TB rats (PCM-TB) for 24-96 h under normoxic (18.6% O2) or hypoxic (5% O2) conditions. Under normoxic condition, there was a decreased AT-1 cell viability in SSM and PCM from the exercise-trained (ET) immune-competent rats, but no difference in PC-3 cell viability in SSM and PCM from ET Nude rats versus the sedentary (SED) group, or in SSM-TB from ET-TB Nude rats versus the SED-TB group. However, there was a decreased PC-3 cell viability in the PCM-TB of the ET-TB group versus SED-TB group. PC-3 cell viability in all conditioned media types was not altered between groups with hypoxia."
Article snippet: Immunohistochemical staining was performed on the Leica Bond-Max autostainer with commercial 5α-reductase 2 (5αR2) antibody (rabbit polyclonal, DPBT-65712RR; Creative Diagnostics, Shirley, NY) diluted 1:1000 with Bond Primary Antibody Diluent (*), with an immunoenzyme peroxidase polymer detection system (*) and diaminobenzidine (DAB) chromogen (*). 
Figure 1. Representative images of immunohistochemical sections stained for 5αR2 (A and B) and caspase-3 (C and D) in the prostate of SED and ET Copenhagen rats, respectively
COA
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