Anti-RPTOR (full length) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Full length protein corresponding to Human Raptor aa 1-379.Sequence: MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWR MKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALE TIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHY NGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQ

Conjugate

Application Notes

WB: 1 μg/ml; ICC/IF: 10 μg/ml;

Alternative Names

RPTOR; regulatory associated protein of MTOR, complex 1; KOG1; Mip1; regulatory-associated protein of mTOR; raptor; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats;

Entrez Gene ID

UniProt ID

Antigen Description

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-389, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

Pathway

AMPK signaling; Cellular responses to stress; HSF1-dependent transactivation; IRS-mediated signalling