Rabbit Anti-Human RPRD1B (a.a. 79-181) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant human RPRD1B protein; Source: E. coli derived, PET30a; Tag: 6*His; Domain: 79-181 aa of BC033629

Conjugate

Application Notes

WB: 1:500-1:2000
IF/ICC: 1:50-1:500

General Notes

This product (Catalog No. DPABH-04756) is a rabbit-derived polyclonal antibody raised against human RPRD1B, targeting the amino acid region 79–181 of the protein. It has been affinity purified by Protein A. C20orf77 interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It is a transcriptional regulator which enhances expression of CCND1. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It also revents RNA polymerase II from reading through the 3 end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
DPABH-04756 is suitable for use in Western blot (WB) (1:500–1:2,000), IF/ICC (1:50–1:500) applications.
DPABH-04756 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

C20orf77; Cell cycle-related and expression-elevated protein in tumor; CREPT; Regulation of nuclear pre-mRNA domain-containing protein 1B

Entrez Gene ID

UniProt ID