Anti-Human RNF2 Polyclonal Antibody (DPABH-23379)
Rabbit Anti-Human RNF2 Polyclonal Antibody for WB, IP, IHC, ChIP
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Immunogen range: Between 150 and 200
Western Blot: 1:2,000 - 1:10,000
Immunoprecipitation: 2 - 5 µg/mg lysate
Immunohistochemistry: 1:500 - 1:2,000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Detection of human and mouse RNF2 by western blot (h&m) and immunoprecipitation (h).
Samples: Whole cell lysate from K562 (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells.
Antibodies: Affinity purified antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human RNF2 by immunohistochemistry.
Sample: FFPE section of human prostate carcinoma.
Antibody: Antibody used at a dilution of 1:1,000 (1µg/ml).
Detection: Vector ImmPACT NovaRED
RNF2; ring finger protein 2; E3 ubiquitin-protein ligase RING2; BAP 1; BAP1; DING
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Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.