Anti-Human RNF2 Polyclonal Antibody (DPABH-23379)

Rabbit Anti-Human RNF2 Polyclonal Antibody for WB, IP, IHC, ChIP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse
Immunogen
Immunogen range: Between 150 and 200
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
Western Blot: 1:2,000 - 1:10,000
Immunoprecipitation: 2 - 5 µg/mg lysate
Immunohistochemistry: 1:500 - 1:2,000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Detection of human and mouse RNF2 by western blot (h&m) and immunoprecipitation (h).
Samples: Whole cell lysate from K562 (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells.
Antibodies: Affinity purified antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human RNF2 by immunohistochemistry.
Sample: FFPE section of human prostate carcinoma.
Antibody: Antibody used at a dilution of 1:1,000 (1µg/ml).
Detection: Vector ImmPACT NovaRED

Target


Alternative Names
RNF2; ring finger protein 2; E3 ubiquitin-protein ligase RING2; BAP 1; BAP1; DING
Entrez Gene ID
UniProt ID

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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