Anti-RNA polymerase II monoclonal antibody (DMAB6568)

Mouse anti-RNA polymerase II monoclonal antibody for WB Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
BSOB-4
Species Reactivity
N/A
Immunogen
Purified RNA polymerase II.
Conjugate
Unconjugated

Target


Alternative Names
RNA polymerase II; RPB1; RPB2; RPB3; RPB4; RPB5

Product Background


Antigen Description
The eukaryotic core RNA polymerase II was first purified using transcription assays. The purified enzyme has typically 10-12 subunits (12 in humans and yeast) and is incapable of specific promoter recognition. Many subunit-subunit interactions are known. RPB3 is involved in RNA polymerase II assembly. A subcomplex of RPB2 and RPB3 appears soon after subunit synthesis. This complex subsequently interacts with RPB1. RPB3, RPB5, and RPB7 interact with themselves to form homodimers, and RPB3 and RPB5 together are able to contact all of the other RPB subunits, except RPB9. Only RPB1 strongly binds to RPB5. The RPB1 subunit also contacts RPB7, RPB10, and more weakly but most efficiently with RPB8. Once RPB1 enters the complex, other subunits such as RPB5 and RPB7 can enter, where RPB5 binds to RPB6 and RPB8 and RPB3 brings in RPB10, RPB 11, and RPB12. RPB4 and RPB9 may enter once most of the complex is assembled. RPB4 forms a complex with RPB7.

Citations


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References


Longerich, S; Tanaka, A; et al. The very 5 ' end and the constant region of Ig genes are spared from somatic mutation because AID does not access these regions. JOURNAL OF EXPERIMENTAL MEDICINE 202:1443-1454(2005).
Dossin, FD; Schenkman, S; et al. Actively transcribing RNA polymerase II concentrates on spliced leader genes in the nucleus of Trypanosoma cruzi. EUKARYOTIC CELL 4:960-970(2005).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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