Anti-RIPK1 monoclonal antibody, clone 8I21 Knockout Validated

Antibody Isotype

IgG2b

Clone

8I21

Species Reactivity

Mouse; Rat; Human

Immunogen

Recombinant full length human RIP protein purified from E.coli (His/ABD-RIP)

Conjugate

Application Notes

Flow Cyt: 1/50 - 1/100; WB: 1/500 - 1/2000.

Alternative Names

RIPK1; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor-interacting serine/threonine-protein kinase 1; RIP; RIP-1; cell death protein RIP; receptor interacting protein; receptor-interacting protein 1; serine/threonine-protein kinase RIP;

Entrez Gene ID

UniProt ID

Antigen Description

Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via Lys-63-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

Pathway

Activated TLR4 signalling, organism-specific biosystem; Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem;