Anti-RAD21 monoclonal antibody, clone 63B422

Antibody Isotype

IgG1

Clone

63B422

Species Reactivity

Human

Immunogen

Synthetic peptide (proprietary-tag) corresponding to Human Rad21 aa 250-650.

Conjugate

Application Notes

IHC-P: 5 μg/ml; WB: 1 μg/ml.

Alternative Names

RAD21; RAD21 homolog (S. pombe); RAD21 (S. pombe) homolog; double-strand-break repair protein rad21 homolog; hHR21; KIAA0078; SCC1; NXP-1; SCC1 homolog; nuclear matrix protein 1; protein involved in DNA double-strand break repair; HR21; MCD1; NXP1; HRAD21

Entrez Gene ID

UniProt ID

Antigen Description

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Pathway

Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem;