General Notes
This product (Catalog No. DPABH-14207) is a rabbit-derived polyclonal antibody raised against human PSMD14, targeting the amino acid region 30–209 of the protein. It has been affinity purified by Protein A. PSMD14 is a protein-coding gene in humans that encodes the 26S proteasome non-ATPase regulatory subunit 14 (also known as POH1 or Rpn11), a critical component of the 19S regulatory particle within the 26S proteasome complex responsible for ATP-dependent protein degradation via the ubiquitin-proteasome pathway. This subunit functions as a zinc metalloprotease with deubiquitinating activity, specifically cleaving 'Lys-63'-linked polyubiquitin chains from substrate proteins to facilitate their processing and degradation, thereby maintaining cellular protein homeostasis. Encoded by a gene located on chromosome 2q24.2, PSMD14 contains a JAMM (JAB1/MPN/MOV34) motif essential for its catalytic function, consisting of conserved charged amino acids that coordinate the zinc ion. Dysregulation of PSMD14 has been associated with cystic fibrosis and Machado-Joseph disease (spinocerebellar ataxia type 3), while in breast cancer, it stabilizes estrogen receptor alpha (ERα) signaling to promote tumor progression.
DPABH-14207 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-14207 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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