Anti-PSMB8 monoclonal antibody, clone FQS25593(C)

Antibody Isotype

IgG

Clone

FQS25593(C)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proteasome 20S LMP7 aa 200 to the C-terminus. The exact sequence is proprietary.Database link: P28062

Conjugate

Application Notes

Flow Cyt: 1/150; IHC-P: 1/250 - 1/500; WB: 1/10000 - 1/50000; ICC/IF: 1/100; IP: 1/40.

Alternative Names

PSMB8; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7); LMP7, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional protease 7); proteasome subunit beta type-8; beta5i; D6S216E; PSMB5i; RIN

Entrez Gene ID

UniProt ID

Antigen Description

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.

Pathway

APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc2 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc2 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc2 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc2 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem.