General Notes
This product (Catalog No. DPABH-04560) is a rabbit-derived polyclonal antibody raised against human PGK1, targeting the amino acid region 1–417 of the protein. This antibody exhibits cross-reactivity with Human PGK1 from mouse and rat. It has been affinity purified by Protein A. The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.
DPABH-04560 is suitable for use in Western blot (WB) (1:5,000–1:50,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:1,000–1:4,000), IF/ICC (1:200–1:800), flow cytometry (FC) (0.25 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-04560 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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