General Notes
This product (Catalog No. DPABH-00608) is a rabbit-derived polyclonal antibody raised against human PGK1, targeting the amino acid region 2–417 of the protein. This antibody exhibits cross-reactivity with Human PGK1 from mouse and rat. It has been affinity purified by Protein A. Phosphoglycerate kinase 1 (PGK1) is a protein-coding gene located on the X chromosome at cytogenetic band Xq21.1 that encodes the enzyme phosphoglycerate kinase 1, a key glycolytic enzyme essential for cellular energy production. This enzyme catalyzes the reversible transfer of a phosphate group from 1,3-bisphosphoglycerate to ADP, yielding 3-phosphoglycerate and ATP, which represents one of the two substrate-level phosphorylation steps in glycolysis that generate ATP without the need for oxidative phosphorylation. Expressed ubiquitously across human tissues, with particularly high levels in the kidney and heart, PGK1 plays a critical role in maintaining energy homeostasis, redox balance, and biosynthetic pathways by regulating ATP and 3-phosphoglycerate levels.
DPABH-00608 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-00608 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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