Rabbit Anti-Human OPA1 (a.a. 261-560) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

Recombinant human OPA1 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 261-560 aa of BC075805

Conjugate

Application Notes

WB: 1:5000-1:50000
IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate

General Notes

This product (Catalog No. DPABH-29045) is a rabbit-derived polyclonal antibody raised against human OPA1, targeting the amino acid region 261–560 of the protein. This antibody exhibits cross-reactivity with Human OPA1 from mouse and rat. It has been affinity purified by Protein A. OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms.
DPABH-29045 is suitable for use in Western blot (WB) (1:5,000–1:50,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate) applications.
DPABH-29045 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

OPA1 gene; OPA 1; EC:3.6.5.5; Dynamin-like GTPase OPA1; short form; Dynamin-like GTPase OPA1; mitochondrial

Entrez Gene ID

UniProt ID