Rabbit Anti-Human OAT (internal) Polyclonal antibody

Background: The booklouse, Liposcelis bostrychophila, is a potent environmental allergen clinically associated with rhino-conjunctivitis and asthma. Despite its known infestation of grain products, anaphylaxis from ingestion of this organism has, to our knowledge, not been previously reported. We present the case of a 44-year-old woman who developed anaphylaxis to ingested oats and rice shown to be contaminated with L. bostrychophila. Objective: The objective was to isolate a distinct antigen from L. bostrychophila implicated in a case of unexplained anaphylaxis. Methods: In vitro studies were obtained for relevant ingested materials and aeroallergens. Skin-prick testing (SPT) was performed with standard extracts, contaminated oats, fresh oats, and crushed L. bostrychophila. Western blots were conducted using subject and control serum to detect specific immunoglobulin E (IgE) against the grains and L. bostrychophila extract. Competitive inhibition immunoblotting was used to assess specificity of IgE binding. Results: In vitro studies and SPT were notable for positive responses to dust mite and flour contaminated by L. bostrychophila, along with contaminated oats. Testing results for fresh oat and rice were negative. Immunoblots that used the subject's serum revealed a strongly positive band in the contaminated oat and rice extracts at 24 kD, whereas dust-mite extract yielded a single 14-kD band. Isolated L. bostrychophila extract also yielded a 24-kD band. Competitive inhibition experiments demonstrated that the 24-kD band in the contaminated oat extract was immunologically distinct from the 14-kD dust-mite band. Conclusion: Our case highlights the importance of considering L. bostrychophila as a potential culprit for unexplained anaphylaxis due to ingested grain products. Given the ubiquitous presence of this insect, we suspect that this may be a more common problem than previously recognized.

Lignocellulosic biomass is the most abundant renewable resource existing, and presently it is considered as a promising feedstock for the synthesis of green chemicals and biofuels for reducing transportation emissions and dependence on fossil fuels. In this research, a TCR (thermo-catalytic reforming) reactor is used to transform lignocellulosic biomass into high quality bio-oils, biochar and syngas. Sugarcane bagasse (SB) and oat hulls (OHS) were processed in the TCR (2 kg/h) at 450 degrees C in the intermediate pyrolysis reactor (kept constant) and varying reforming temperature between 500 and 700 degrees C. This paper studies the conversion potential of SB and OHS into sustainable bioproducts at different reforming temperatures. TCR oils contained the highest calorific value of 34.9 MJ/kg from SB and 35.0 MJ/kg from OHS at 700 degrees C which makes the bio-oil suitable to be applied as fuel for engines when compared to bio-oils produced from other pyrolysis technologies. SB and OHS pyrolysis gas at 700 degrees C contained higher H-2 yield 30.9 vol% and 31.6 vol%, respectively. The highest gas caloric values of SB and OHS were 18.4 MJ/kg and 18.8 MJ/kg at the same reforming temperatures, respectively. Overall, the TCR results of SB and OHS are promising for waste conversion into sustainable fuels.