Anti-Nanog monoclonal antibody (DCABH-193)

Rabbit Anti-Nanog monoclonal antibody for WB, ICC/IF, IHC, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TD16-81
Species Reactivity
Human, Mouse
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog

Citations


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References


Kol'tsova, AM; Gordeeva, OF; et al. Comparative Characteristics of New Human Embryonic Stem Cell Lines SC5, SC6, SC7, and SC3a. RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY 42:212-225(2011).
Hua, JL; Pan, SH; et al. Derivation of male germ cell-like lineage from human fetal bone marrow stern cells. REPRODUCTIVE BIOMEDICINE ONLINE 19:99-105(2009).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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