Anti-Nanog monoclonal antibody (DCABH-193)

Rabbit Anti-Nanog monoclonal antibody for WB, ICC/IF, IHC, FC Datasheet

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Host Species
Antibody Isotype
Species Reactivity
Human, Mouse
Recombinant protein


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples

Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody


Alternative Names
Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog


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Sharivkin, R; Walker, MD; et al. Proteomics-based Dissection of Human Endoderm Progenitors by Differential Cell Capture on Antibody Array. MOLECULAR & CELLULAR PROTEOMICS 11:586-595(2012).
Liu, BG; Badeaux, MD; et al. Nanog1 in NTERA-2 and Recombinant NanogP8 from Somatic Cancer Cells Adopt Multiple Protein Conformations and Migrate at Multiple M.W Species. PLOS ONE 9:-(2014).

Custom Antibody Labeling

We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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