Rabbit Anti-NOS1 Polyclonal Antibody (DPABH-00343)
Rabbit Anti-Human NOS1 Polyclonal Antibody for WB, IF
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Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1).
WB: 1:500 - 1:2000; IF: 1:20 - 1:50
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using NOS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
NOS1; nitric oxide synthase 1 (neuronal); NOS; bNOS; nNOS; IHPS1
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Ferrusola, CO; Fernandez, LG; et al. Effect of Cryopreservation on Nitric Oxide Production by Stallion Spermatozoa. BIOLOGY OF REPRODUCTION 81:1106-1111(2009).
Northington, FJ; Koehler, RC; et al. Nitric oxide synthase 1 and nitric oxide synthase 3 protein expression is regionally and temporally regulated in fetal brain. DEVELOPMENTAL BRAIN RESEARCH 95:1-14(1996).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.