Anti-NARF (aa 156-349) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant fragment, corresponding to a region within internal amino acids 156-349 of Human NARF (Uniprot ID: Q9UHQ1).

Conjugate

Application Notes

WB: 1/500 - 1/3000; ICC/IF: 1/100 - 1/1000.

Alternative Names

NARF; nuclear prelamin A recognition factor; DKFZp434G0420; FLJ10067; IOP2; iron only hydrogenase like protein 2; iron-only hydrogenase-like protein 2; prenyl-dependent prelamin A binding protein;

Entrez Gene ID

UniProt ID

Antigen Description

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.