Rabbit Anti-Human MDH1 (internal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

Fusion protein corresponding to a region derived from internal residues of human malate dehydrogenase 1, NAD

Conjugate

Application Notes

WB: 1:500-1:2000

General Notes

This product (Catalog No. DPABH-27287) is a rabbit-derived IgG polyclonal antibody raised against human MDH1. This antibody exhibits cross-reactivity with MDH1 from mouse. It has been affinity purified by protein A. This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
DPABH-27287 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
DPABH-27287 is supplied as a liquid formulation in pH7.4 PBS, 0.05% sodium azide, 40% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375

Entrez Gene ID

UniProt ID