Anti-Human MAP2K1 Polyclonal Antibody (DPABH-04774)

Rabbit Anti-Human MAP2K1 Polyclonal Antibody for WB, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Immunogen range: Between 343 and 393
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
Western Blot: 1:2,000 - 1:10,000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Detection of human MEK1 by western blot and immunoprecipitation.
Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.
Antibodies: Affinity purified antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MEK1 was efficiently immunoprecipitated by rabbit anti-MEK1 antibody, which recognizes an upstream epitope.
Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human MEK1 by immunohistochemistry.
Sample: FFPE section of human breast carcinoma (left) and colon carcinoma (right).
Antibody: Antibody used at a dilution of 1:1,000 (1µg/ml).
Detection: DAB

Target


Alternative Names
MAP2K1; mitogen-activated protein kinase kinase 1; CFC3; MEK1; MKK1; MAPKK1
Entrez Gene ID
UniProt ID

Citations


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References


MacDonald, TJ; Brown, KM; et al. Expression profiling of medulloblastoma: PDGFRA and the RAS/MAPK pathway as therapeutic targets for metastatic disease. NATURE GENETICS 29:143-152(2001).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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