Anti-Human MAP2K1 Polyclonal Antibody (DPABH-04774)
Rabbit Anti-Human MAP2K1 Polyclonal Antibody for WB, IHC
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Immunogen range: Between 343 and 393
Western Blot: 1:2,000 - 1:10,000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Detection of human MEK1 by western blot and immunoprecipitation.
Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.
Antibodies: Affinity purified antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MEK1 was efficiently immunoprecipitated by rabbit anti-MEK1 antibody, which recognizes an upstream epitope.
Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human MEK1 by immunohistochemistry.
Sample: FFPE section of human breast carcinoma (left) and colon carcinoma (right).
Antibody: Antibody used at a dilution of 1:1,000 (1µg/ml).
MAP2K1; mitogen-activated protein kinase kinase 1; CFC3; MEK1; MKK1; MAPKK1
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MacDonald, TJ; Brown, KM; et al. Expression profiling of medulloblastoma: PDGFRA and the RAS/MAPK pathway as therapeutic targets for metastatic disease. NATURE GENETICS 29:143-152(2001).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.