Anti-Human Phospho MEK1(T286) Polyclonal Antibody (DPABH-01585)

Rabbit Anti-Human Phospho MEK1(T286) Polyclonal Antibody for IP, IHC

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse
Immunogen
Surrounding threonine 286
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
Immunoprecipitation: 2 - 10 µg/mg lysate
Immunohistochemistry: 1:1,000 - 1:5,000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Detection of human Phospho-MEK1 (T286) by immunohistochemistry.
Sample: FFPE section of human breast carcinoma.
Antibody: Antibody used at a dilution of 1:5,000 (0.2µg/ml).
Detection: DAB
Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry.
Sample: FFPE section of mouse renal cell carcinoma.
Antibody: Antibody used at a dilution of 1:1,000 (1µg/ml).
Detection: DAB
Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP).
Samples: 50 µg HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded.
Detection: Chemiluminescence with exposure time of 10 seconds.

Target


Alternative Names
MAP2K1; mitogen-activated protein kinase kinase 1; CFC3; MEK1; MKK1; MAPKK1
Entrez Gene ID
UniProt ID

Citations


Have you cited DPABH-01585 in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


MacDonald, TJ; Brown, KM; et al. Expression profiling of medulloblastoma: PDGFRA and the RAS/MAPK pathway as therapeutic targets for metastatic disease. NATURE GENETICS 29:143-152(2001).

Online Inquiry

Name:
Phone: *
E-mail Address: *
Service & Products Interested: *
Project Description:
Verification Code: * Please input "diagnostics" as verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket