Anti-Lectin 1 (native) polyclonal antibody

Purpose: The mannose-binding lectin pathway of innate immunity is part of the first line of defense against microorganisms. Variations in mannose-binding lectin levels or activity are caused by single-nucleotide polymorphisms in the promoter region. Mannose-binding lectin may play a role as a phase reactant due to inflammatory processes related to cancer disease. Such inflammatory processes are well known in colorectal cancer and have been shown to be independent of stage of disease. We aimed to investigate whether profile of mannose-binding lectin-2 -221G>C genotyping may be associated with the risk of colorectal cancer. Materials and Methods: The study group consisted of 107 patients were diagnosed with histologically confirmed cancer of the colon or rectum. The control group consisted of 99 were selected among healthy people. Genomic DNA of control and patients was extracted from whole blood using High Pure PCR template preparation kit. Genotyping of mannose-binding lectin-2 polymorphisms were detected by using a mannose-binding lectin-2 mutation detection kit in real-time PCR. Chi-square or Fisher's Exact Tests were used to evaluate the distribution of the -221G>C genotypes among the patients and control subjects. Associations between -221G>C genotype and colorectal cancer risk were analyzed by binary logistic regression. Results: Logistic regression analyses showed that the mannose-binding lectin-2 GC genotype was associated with a significantly increased risk of colorectal cancer. The odds ratio of colorectal cancer for the mannose-binding lectin-2 GC genotype was 2.095 (95% CI=1.76-3.731, P=0.012) compared with the control group. Conclusion: These results suggest that mannose-binding lectin-2 G/C allele gene polymorphisms may be associated with genetic susceptibility of colorectal cancer.

Mannose-binding lectin (MBL) is a pattern recognition receptor of the lectin pathway of complement system. MBL binds to carbohydrates on microorganism's surfaces leading to complement activation, opsonization and phagocytosis. Polymorphisms in the MBL gene (MBL2) are associated with variations on MBL serum levels and with the susceptibility to various infectious and autoimmune diseases. The involvement of the lectin pathway in rheumatoid arthritis (RA) has been demonstrated by several studies and although MBL has been considered to have a dual role in the pathogenesis of the disease, the association between MBL and RA remains inconclusive. In an attempt to clarify this relationship, we developed this short review summarizing accumulated evidences in regard to MBL and RA and a meta-analysis to evaluate the influence of MBL2 polymorphisms on the susceptibility to RA. Among a total of 217 articles that were identified following a predefined search strategy on PubMed, Scopus, Scielo, EMBASE and Cochrane databases, only 13 met all inclusion criteria and were included in the meta-analysis. Data assessment was conducted by three independent investigators and presented in odds ratio (OR) and 95% confidence intervals (CIs) using forest plot charts. Both heterogeneity and publication bias were analyzed. The results of the meta-analysis evidenced that MBL2 low producing 00 and XX genotypes do not confer higher risk to RA, even when data were analyzed according to cohort's ethnicity. Further studies are needed in order to clarify the importance of other genes of the lectin pathway in the pathogenesis of RA. (C) 2015 Elsevier Ltd. All rights reserved.