Anti-LIN28A monoclonal antibody

Antibody Isotype

IgG2b

Clone

HU2197

Species Reactivity

Human

Immunogen

Recombinant fragment within Human Lin28 aa 1-180. The exact sequence is proprietary.Database link: Q9H9Z2

Conjugate

Unconjugated

Application Notes

IHC-P: 1/100 - 1/1000; WB: 1/500 - 1/3000; ICC/IF: 1/100 - 1/1000; Flow Cyt: 1/50.

Alternative Names

LIN28A; lin-28 homolog A (C. elegans); lin 28 homolog (C. elegans) , LIN28; protein lin-28 homolog A; CSDD1; FLJ12457; LIN 28; ZCCHC1; RNA-binding protein LIN-28; zinc finger, CCHC domain containing 1; zinc finger CCHC domain-containing protein 1; LIN28;

Entrez Gene ID

79727

UniProt ID

Q9H9Z2

Antigen Description

Acts as a translational enhancer, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5-GGAG-3 motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5-GGAG-3 motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More