Rabbit Anti-Human LDHC Polyclonal Antibody

Human lactate dehydrogenase (LDH) has attracted attention as a potential target for cancer therapy and contraception. In this study, we reconstituted human lactic acid fermentation in Saccharomyces cerevisiae, with the goal of constructing a yeast cell-based LDH assay system. pdc null mutant yeast (mutated in the endogenous pyruvate decarboxylase genes) are unable to perform alcoholic fermentation; when grown in the presence of an electron transport chain inhibitor, pdc null strains exhibit a growth defect. We found that introduction of the human gene encoding LDHA complemented the pdc growth defect; this complementation depended on LDHA catalytic activity. Similarly, introduction of the human LDHC complemented the pdc growth defect, even though LDHC did not generate lactate at the levels seen with LDHA. In contrast, the human LDHB did not complement the yeast pdc null mutant, although LDHB did generate lactate in yeast cells. Expression of LDHB as a red fluorescent protein (RFP) fusion yielded blebs in yeast, whereas LDHA-RFP and LDHC-RFP fusion proteins exhibited cytosolic distribution. Thus, LDHB exhibits several unique features when expressed in yeast cells. Because yeast cells are amenable to genetic analysis and cell-based high-throughput screening, our pdc/LDH strains are expected to be of use for versatile analyses of human LDH.

Soil deformation and unsaturated transient water flow in swelling soil on a laboratory scale is predicted using a one-dimensional numerical model. The model is based on a soil water flow equation and extended to soil deformation using Lagrangian description (LD). The specific features of the model are inclusion of an overburden component in the total potential of the flow equation, introduction of a shrinkage-swelling characteristic (SSC) known as a third soil hydraulic function, and two-dimensional analysis of soil deformation using a geometry factor. This paper describes an evaluation of the model, which was previously verified with a case of shrinkage of marine clay soil, with a data set from an infiltration experiment performed under swelling conditions. Soil hydraulic properties such as moisture retention characteristic (MRC), hydraulic conductivity function (K(h)) and SSC were derived from simultaneous measurement of volumetric moisture content (VMC). bulk density and pressure heads in a framework of the Eulerian description (ED). Two different Ii(h) relationships, with respect to moving solid particles, were obtained from both the LD and ED. Simulation was performed using two different hydraulic conductivities, i.e., Eulerian descriptive hydraulic conductivity (EDHC) and Lagrangian descriptive hydraulic conductivity (LDHC). Model performance was evaluated using four different statistical parameters representing the goodness of model predictions on the transient variations of soil moisture contents. Results of the evaluation reveal that the model predicts reasonably well the moisture content changes, although the use of LDHC gives slightly better results than the EDHC. Overprediction of moisture contents by the simulation model especially near the bottom of the sample is attributed to the incomplete dissipation of swelling pressure during expansion of the soil material. As a result, the soil shows anisotropic swelling although it was forced to deform in the one-dimensional vertical direction by experimental design. Unlike other one-dimensional consolidation models, the model can be used for two-dimensional analysis of soil deformation in porous media with either self-weight or external load application, at either shrinking or swelling phase and under both saturated and unsaturated flow conditions. (C) 1999 Elsevier Science B.V. All rights reserved.