Anti-NOX4 monoclonal antibody (CABT-38500RH)

Rabbit Anti-NOX4 monoclonal antibody for WB, ICC, IHC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TZ1325
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000; ICC: 1:50-1:100; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1,000 dilution.
Fig2: ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NOX4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NOX4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-NOX4 antibody. Counter stained with hematoxylin.

Target


Alternative Names
Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH

Citations


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References


Kern, G; Mair, SM; et al. Tacrolimus Increases Nox4 Expression in Human Renal Fibroblasts and Induces Fibrosis-Related Genes by Aberrant TGF-Beta Receptor Signalling. PLOS ONE 9:-(2014).
Altenhofer, S; Kleikers, PWM; et al. The NOX toolbox: validating the role of NADPH oxidases in physiology and disease. CELLULAR AND MOLECULAR LIFE SCIENCES 69:2327-2343(2012).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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