Anti-HSPH1 monoclonal antibody (CABT-33542RH)

Rabbit Anti-HSPH1 monoclonal antibody for WB, ICC/IF, IHC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TE96-17
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Hsp105 on different lysates using anti-Hsp105 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7; Lane 2: Hela
Fig2: ICC staining Hsp105 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Hsp105 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Hsp105 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.

Target


Alternative Names
Antigen NY CO 25; Antigen NY-CO-25; DKFZp686M05240; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta

Citations


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References


Mitchell, LA; Nixon, B; et al. Analysis of chaperone proteins associated with human spermatozoa during capacitation. MOLECULAR HUMAN REPRODUCTION 13:605-613(2007).
Buttner, P; Mosig, S; et al. Exercise affects the gene expression profiles of human white blood cells. JOURNAL OF APPLIED PHYSIOLOGY 102:26-36(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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