Anti-HSPH1 monoclonal antibody (CABT-33542RH)
Rabbit Anti-HSPH1 monoclonal antibody for WB, ICC/IF, IHC, IP
Datasheet
Specifications
Host Species
Rabbit
Antibody Isotype
IgG
Clone
TE96-17
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated
Applications
Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Data Examples
Fig1: Western blot analysis of Hsp105 on different lysates using anti-Hsp105 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7;
Lane 2: Hela
Fig2: ICC staining Hsp105 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Hsp105 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Hsp105 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
Target
Alternative Names
Antigen NY CO 25; Antigen NY-CO-25; DKFZp686M05240; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta
Citations
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References
Buttner, P; Mosig, S; et al. Exercise affects the gene expression profiles of human white blood cells. JOURNAL OF APPLIED PHYSIOLOGY 102:26-36(2007).
Lamore, SD; Wondrak, GT; et al. Zinc pyrithione impairs zinc homeostasis and upregulates stress response gene expression in reconstructed human epidermis. BIOMETALS 24:875-890(2011).
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Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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