Anti-Human CHGA monoclonal antibody (DMAB5562RH)
Rabbit Anti-Human CHGA monoclonal antibody for WB, ICC/IF, IHC
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Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of Chromogranin A on PC-12 cells lysates using anti-Chromogranin A antibody at 1/1,000 dilution.
Fig2: ICC staining Chromogranin A in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
beta Granin; betagranin (N-terminal fragment of chromogranin A); catestatin; CgA; CHG A; Chga
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Portela-Gomes, GM; Gayen, JR; et al. The importance of chromogranin A in the development and function of endocrine pancreas. REGULATORY PEPTIDES 151:19-25(2008).
Kiflemariam, S; Andersson, S; et al. Scalable In Situ Hybridization on Tissue Arrays for Validation of Novel Cancer and Tissue-Specific Biomarkers. PLOS ONE 7:-(2012).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.