Animals were immunized with human peripheral blood monocytes. Spleen cells were fused with P3U1 myeloma cells. The antibody reacts with human class II antigens (probably HLA DR and DQ). The antibody precipitates a heterodimer of 33000 and 29000 daltons. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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COA
Certificate of Analysis
