Anti-Glyoxal polyclonal antibody

In this work, gold nanoparticles (AuNPs) and graphene oxide (GO) were successfully incorporated separately into the poly(vinyl) alcohol (PVA) crosslinked composite films. Glyoxal and/or glutaraldehyde (GA) were employed as a crosslinking agent in film fabrication. Through the analytical approach, the success in imparting the crosslink toward PVA by which the relative transmittance intensity of the hydroxyl group is subsided to a greater extent has verified by the FTIR analysis. The dispersion of AuNPs and GO in the PVA crosslinked composites have improved the mechanical or physical properties (tensile strength, Young's modulus value, water vapor transmission rate, water solubility) and making them promising candidates for food packaging application. The outcome of the agar disk diffusion test reveals that AuNPs incorporated PVA crosslinked composite film possess a greater capability with the formation of the larger inhibition zone to prevent the microbial contamination than GO-PVA crosslinked composite film. Banana shelf life has qualitatively improved with PVA-glyoxal-AuNPs composite film for food preservation and affirmed the uses in food packaging applications.

Herein, a simple and sensitive method was successfully developed for the extraction and quantification of seven carbonyl compounds (acetaldehyde, propanal, butanal, pentanal, hexanal, glyoxal, methylglyoxal) in a number of wastewater samples previously treated by ozonation process. The compounds were initially derivatized (derivatizing reagent (PFBHA)) and then extracted by a hollow-fiber liquid-phase microextraction method (HF-LPME) in the aqueous sample solution. Afterwards, the extraction solvent was withdrawn from the fiber and injected to a gas chromatograph equipped with electron capture detection (GC-ECD). Factors affecting the extraction efficiency of the whole procedure were optimized using a univariate approach. Under the optimal conditions obtained (sample solution pH, 4; organic extraction solvent, dihexylether; stirring rate, 1000 rpm; no salt addition; and extraction time of 40 min), limits of quantification were 0.5-0.8 mu g/l for the studied carbonyl compounds. Dynamic linear ranges were 0.5-100 mu g/l for five targets (acetaldehyde, propanal, butanal, pentanal and hexanal) and 0.8-80 mu g/l for two targets (glyoxal and methylglyoxal). The relative standard deviations (RSDs %) representing the precision of the method were in the range of 6.4-11.9 based on the average of three measurements. Accuracy of the method was also tested by the relative recovery experiments on spiked samples, with results ranging from 85 to 113%. Finally, the method could be used for routine screening of carbonyl compounds in complex matrices.