Type O, Rh- human erythrocytes and purified human glycophorin A
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
OO-4
Cell Line Description
Animals were immunized with type O, Rh- human erythrocytes and purified human glycophorin A. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
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