GeneralInformation
Immunogen
Double negative (DN) sheep T cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with double negative (DN) sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(50%FBS), add 5% (v/v) DMSO
Citations
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Langerak, AW; Wolvers-Tettero, ILM; et al. Immunophenotypic and immunogenotypic characteristics of TCR gamma delta(+) T cell acute lymphoblastic leukemia. LEUKEMIA 13:206-214(1999).
Braylan, RC; Orfao, A; et al. Optimal number of reagents required to evaluate hematolymphoid neoplasias: Results of an international consensus meeting. CYTOMETRY 46:23-27(2001).
COA
Certificate of Analysis
