Mouse Anti-Sheep Gamma/Delta T Cell Receptor Hybridoma [97E] (CSC-H0550)

This hybridoma produces mAbs (IgG1, kappa light chain) against sheep Gamma/Delta T cell Receptor

General Information


Immunogen
Double negative (DN) sheep T cells
Isotype
IgG1, kappa light chain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
97E
Cell Line Description
Animals were immunized with double negative (DN) sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
IMDM with 2 mM L-glutamine, supplemented with 10% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium(50%FBS), add 5% (v/v) DMSO

Target


Introduction
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes (or T cells) that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. The binding between TCR and antigen is of relatively low affinity and is degenerate: that is, many TCR recognize the same antigen and many antigens are recognized by the same TCR.

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