Anti-GTF2F1 monoclonal antibody, clone 3B26

Antibody Isotype

IgG

Clone

3B26

Species Reactivity

Human

Immunogen

Full length native protein (purified) (Human).

Conjugate

Alternative Names

GTF2F1; general transcription factor IIF, polypeptide 1, 74kDa; general transcription factor IIF, polypeptide 1 (74kD subunit); general transcription factor IIF subunit 1; BTF4; RAP74; TF2F1; TFIIF; TFIIF-alpha; transcription initiation factor RAP74; gene

Entrez Gene ID

UniProt ID

Antigen Description

The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.

Pathway

Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem;