Anti-GPR77 monoclonal antibody Made to order

Name: Anti-GPR77 monoclonal antibody
SKU: DCABH-11799
Rating: 5 (1 reviews)

Rabbit anti-Human GPR77 monoclonal antibody for WB, ELISA

Write a review

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

A synthetic peptide of human GPR77 is used for rabbit immunization.

Conjugate

Unconjugated

Target

Alternative Names

GPR77; G protein-coupled receptor 77; C5a anaphylatoxin chemotactic receptor C5L2; C5L2; G-protein coupled receptor 77; G protein-coupled receptor C5L2; GPF77;

Entrez Gene ID

27202

UniProt ID

Q9P296

Product Background

Antigen Description

The anaphylatoxins C3a (see MIM 120700), C4a (see MIM 120810), and C5a are cationic fragments generated during the complement cascade that participate in host defense. In the case of inappropriate complement activation, anaphylatoxins may be involved in autoimmunity and sepsis. C5L2 is coexpressed with the C5a receptor, C5AR (C5R1; MIM 113995), on polymorphonuclear neutrophils and may modulate C5AR activity (Gerard et al., 2005 [PubMed 16204243]).

Pathway

Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem.

Citations

Have you cited DCABH-11799 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Recombinant C3adesArg/acylation stimulating protein (ASP) is highly bioactive: A critical evaluation of C5L2 binding and 3T3-L1 adipocyte activation

MOLECULAR IMMUNOLOGY

Authors: Cui, Wei; Lapointe, Marc; Gauvreau, Danny; Kalant, David; Cianflone, Katherine

Abstract

C5L2 is a recently identified receptor for C5a/C5adesArg, C3a and C3adesArg (ASP). C5a/C5adesArg bind with high affinity, with no identified activation. By contrast, some studies demonstrate C3a/ASP binding/activation to C5L2; others do not. Our aim is to critically evaluate ASP/C3adesArg-C5L2 binding and bioactivity. Cell-associated fluorescent-ASP (FI-ASP) binding to C5L2 increased from transiently transfected < stably transfected

C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a

NATURE

Authors: Chen, Nien-Jung; Mirtsos, Christine; Suh, Daniel; Lu, Yong-Chen; Lin, Wen-Jye; McKerlie, Colin; Lee, Taeweon; Baribault, Helene; Tian, Hui; Yeh, Wen-Chen

Abstract

Complement-derived anaphylatoxins regulate immune and inflammatory responses through G-protein-coupled receptor ( GPCR)mediated signalling(1-4). C5L2 ( also known as GPR77) is a relatively new GPCR thought to be a non-signalling receptor binding to C5a, on the basis of sequence information and experimental evidence(5-7). Here we show, using gene targeting, that C5L2 is required to facilitate C5a signalling in neutrophils, macrophages and fibroblasts in vitro. Deficiency of C5L2 results in reduced inflammatory cell infiltration, suggesting that C5L2 is critical for optimal C5a-mediated cell infiltration in certain in vivo settings. C5L2 is also involved in optimizing C3a-induced signals. Furthermore, like mice incapable of C3a/complement 3a receptor (C3aR) signalling(4,8,9), C5L2-deficient mice are hypersensitive to lipopolysaccharide (LPS)-induced septic shock, show reduced ovalbumin (OVA)-induced airway hyperresponsiveness and inflammation, and are mildly delayed in haematopoietic cell regeneration after gamma-irradiation. Our data indicate that C5L2 can function as a positive modulator for both C5a- and C3a-anaphylatoxin-induced responses.