First report of Colletotrichum truncatum associated with anthracnose disease on tuberose (Polianthes tuberosa) in India
CROP PROTECTION
Authors: Mahadevakumar, S.; Chandana, C.; Janardhana, G. R.
Abstract
Tuberose (Polianthes tuberosa) is an important commercial flower crop grown in Karnataka for its beautiful and fragrant cut flowers. Recently, an association of anthracnose disease (incidence ranged from 18 to 27%) characterized by the dark concentric sunken necrotic lesions with spore mass in the acervuli on leaves and peduncles of Tuberose was observed in a field survey conducted during October 2015 to March 2016. The pathogen was isolated on PDA medium. The fungal colony on PDA was grayish to dark gray. Conidia were falcate, one-celled, hyaline. Based on the micro-morphological, and cultural characteristics the pathogen was identified as Colletotrichum truncatum. The ITS-rDNA, GAPDH and beta-tubulin sequences of the pathogen were sequenced using ITS1/ITS4, GDF1/GDR1, T1/Bt2b primer pairs. nBLAST search and phylogenetic analysis confirmed that the pathogen was C. truncatum. Koch's postulates were conducted on 45-day-old tuberose plants by foliar application of conidial suspension of C. truncatum. Development of typical anthracnose disease was recorded after 18 days of post-inoculation and the pathogen's identity was confirmed by re-isolation and identification. The anthracnose disease associated with Tuberose is a major constraint for the production of quality cut flowers. This is the first report of C. truncatum causing anthracnose on Tuberose in India.
Contribution of rare inherited and de novo variants in 2,871 congenital heart disease probands
NATURE GENETICS
Authors: Jin, Sheng Chih; Homsy, Jason; Zaidi, Samir; Lu, Qiongshi; Morton, Sarah; DePalma, Steven R.; Zeng, Xue; Qi, Hongjian; Chang, Weni; Sierant, Michael C.; Hung, Wei-Chien; Haider, Shozeb; Zhang, Junhui; Knight, James; Bjornson, Robert D.; Castaldi, Christopher; Tikhonoa, Irina R.; Bilguvar, Kaya; Mane, Shrikant M.; Sanders, Stephan J.; Mital, Seema; Russell, Mark W.; Gaynor, J. William; Deanfield, John; Giardini, Alessandro; Porter, George A., Jr.; Srivastava, Deepak; Lo, Cecelia W.; Shen, Yufeng; Watkins, W. Scott; Yandell, Mark; Yost, H. Joseph; Tristani-Firouzi, Martin; Newburger, Jane W.; Roberts, Amy E.; Kim, Richard; Zhao, Hongyu; Kaltman, Jonathan R.; Goldmuntz, Elizabeth; Chung, Wendy K.; Seidman, Jonathan G.; Gelb, Bruce D.; Seidman, Christine E.; Lifton, Richard P.; Brueckner, Martina
Abstract
Congenital heart disease (CHD) is the leading cause of mortality from birth defects. Here, exome sequencing of a single cohort of 2,871 CHD probands, including 2,645 parent-offspring trios, implicated rare inherited mutations in 1.8%, including a recessive founder mutation in GDF1 accounting for similar to 5% of severe CHD in Ashkenazim, recessive genotypes in MYH6 accounting for similar to 11% of Shone complex, and dominant FLT4 mutations accounting for 2.3% of Tetralogy of Fallot. De novo mutations (DNMs) accounted for 8% of cases, including similar to 3% of isolated CHD patients and similar to 28% with both neurodevelopmental and extra-cardiac congenital anomalies. Seven genes surpassed thresholds for genome-wide significance, and 12 genes not previously implicated in CHD had > 70% probability of being disease related. DNMs in similar to 440 genes were inferred to contribute to CHD. Striking overlap between genes with damaging DNMs in probands with CHD and autism was also found.
COA
Publication 
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