Anti-FITC polyclonal antibody [Biotin]

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 mu m) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.

Two new coordination polymers (CPs), namely [Cu(4-bptzp)(1,3-BDC)](n)(1, 1,3-H2BDC& x202f;=& x202f;1,3-benzenedicarboxylic acid, 4-bptzp& x202f;=& x202f;1,4-bis(5-(4-pyridyl)tetrazolyl)propane) and [Co(4-bptzh)(1,3-BDC)](n)(2, 4-bptzh = 1,4-bis(5-(4-pyridyl)tetrazolyl)hexane) were prepared by hydrothermal reactions of the corresponding metal salts with the 1,3-H2BDC in the presence of different flexible bis(pyridyl-tetrazole) ligands. The inhibitory effect of compounds1and2on SK-OV-3 human ovarian cancer cells was assessed. Firstly, the CCK-8 assay was performed and the anti-proliferation activity of compounds1and2was assessed. Then, the Annexin V-FITC/PI apoptosis assay was performed to detect the apoptosis of the cancer cells in flow cytometry.