Anti-FHL2 (aa 217-279) polyclonal antibody

In oder to study effect of BRD7 gene on NPC cell line CNE1, BRD7 was introduced into CNE1 cells by liposome transfection. BRD7 transfected cells were resulted in a declined growth curve. To account for the mechanism of this gene fimction on CNE1, two-dimensional polyacrylamide gel eletrophoresis(2-D PAGE) and MALDI-TOF were performed. After image analysis and MALDI-TOF identification, 19 differential expression proteins were identified. These proteins included BCCIP (BRCA2 and CDKN1A (p21 (Waf1 /Cip1)), FHL2 (four and a half LIM domains 2), Chloride channel regulatory protein, Hin-1 (high-in-normal-1), WISP-1 (connective tissue growth factor related protein), SREC-4(scavenger receptor expressed by endothelial cells-2), folate receptor, which involved in transcription regulation, adherence and so on. The study extended the research field of BRD7 and reinforced the evidences that BRD7 act as a NPC-related candidate suppressor gene.

The standard procedure for the osteogenic differentiation of multipotent stem cells is treatment of a confluent monolayer with a cocktail of dexamethasone (Dex), ascorbic acid (Asc) and beta-glycerophosphate (beta-Gly). This review describes the effects of these substances on intracellular signaling cascades that lead to osteogenic differentiation of bone marrow stroma-derived stem cells. We conclude that Dex induces Runx2 expression by FHL2/beta-catenin-mediated transcriptional activation and that Dex enhances Runx2 activity by upregulation of TAZ and MKP1. Asc leads to the increased secretion of collagen type I (Col1), which in turn leads to increased Col1/alpha(2)beta(1) integrin-mediated intracellular signaling. The phosphate from beta-Gly serves as a source for the phosphate in hydroxylapatite and in addition influences intracellular signaling molecules. In this context we give special attention to the differences between dystrophic and bone-specific mineralization.