Rabbit Anti-Human FBXL2 Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

A synthesized peptide derived from human FBXL2.

Conjugate

Application Notes

IF/ICC 1:100-1:500
WB 1:500-1:2000

General Notes

This product (Catalog No. DPABH-03890) is a rabbit-derived polyclonal antibody raised against human FBXL2. This antibody exhibits cross-reactivity with FBXL2 from mouse. It has been affinity purified by Protein A. Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity).
DPABH-03890 is suitable for use in IF/ICC 1 (100-1:500), WB 1 (500-1:2000) applications.
DPABH-03890 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID