General Notes
This product (Catalog No. DPABH-03725) is a rabbit-derived polyclonal antibody raised against human ENDOG, targeting the amino acid region 63–297 of the protein. This antibody exhibits cross-reactivity with Human ENDOG from mouse and rat. It has been affinity purified by Protein A. Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.
DPABH-03725 is suitable for use in Western blot (WB) (1:2,000–1:16,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:400–1:1,600) applications.
DPABH-03725 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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