Anti-EHMT2 (full length) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Mouse; Rat; Human

Immunogen

Recombinant full length protein corresponding to Human KMT1C/ G9a aa 911-1210 (C terminal).Sequence: VGNRAIRTEK IICRDVARGY ENVPIPCVNG VDGEPCPEDY KYISENCETS TMNIDRNITH LQHCTCVDDC SSSNCLCGQL SIRCWYDKDG RLLQEFNKIE PPLIFECNQA CSCWRNCKNR VVQSGIKVRL QLYRTAKMGW

Conjugate

Application Notes

IHC-P: 1/50 - 1/200; WB: 1/500 - 1/2000;

Alternative Names

EHMT2; euchromatic histone-lysine N-methyltransferase 2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30; histone-lysine N-methyltransferase EHMT2; H3-K9-HMTase 3; G9A histone methyltransferase; HLA-B associated transcript 8; lysine N-methyltransferase 1C; ankyrin

Entrez Gene ID

UniProt ID

Antigen Description

Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of Lys-56 of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Pathway

Cellular Senescence; Gene Expression; Lysine degradation; RNA Polymerase I Transcription