Magic™ Anti-Digoxigenin polyclonal antibody [HRP]

Scientists first extracted a steroid called Digoxigenin (DIG) from Digitalis purpurea. Since then, it has been widely used in biological research. It is very specific and sensitive, and scientists can detect when it binds to antibodies. One of the key benefits of DIG is its effectiveness in detecting target molecules with precision. In experiments, scientists usually connect DIG to nucleic acids or oligonucleotide probes. They then use anti-DIG to detect these probes, creating visible signals with enzyme-linked reactions. This approach is often used in methods like in situ hybridization (ISH), Southern blotting, and Northern blotting, which are important for accurately finding and measuring nucleic acids. In ISH, DIG is linked to ribonucleoside triphosphates, allowing the production of RNA probes that can selectively attach to target mRNA in preserved tissue samples. This technique offers valuable insights into gene expression patterns at the cellular level. Furthermore, the exceptional sensitivity of DIG allows for the identification of low-abundance targets, making it an essential tool for investigating gene regulation and expression across various biological scenarios.

Figure 1. Detection of DIG-labeled nucleic acid probes with BCIP/NBT or AMPPD
(Source: Green MR, et al. 2022)

DIG, being a non-radioactive marker, provides several key benefits compared to conventional radioactive probes, such as improved safety, stability, and heightened sensitivity. Its lack of radioactivity removes the safety risks linked to radioactive isotopes, which makes it a favored option in numerous research settings. Additionally, DIG-labeled probes maintain their stability over time, enabling experiments to be conducted over longer periods without a notable decrease in signal strength. This characteristic is particularly important when dealing with low-abundance targets, where enhancing the signal is essential for accurate detection.

Beyond its role in nucleic acid detection, DIG has proven effective in the study of proteins as well. Its adaptability enables it to be linked with different proteins for use in immunoassays, which aids in identifying and measuring specific antigens. DIG can also be utilized in immunodetection techniques, where it attaches to antibodies to enhance signal amplification and facilitate detection.

Anti-DIG polyclonal antibody