Anti-DKK1 monoclonal antibody, clone 252246

Antibody Isotype

IgG2b

Clone

252246

Species Reactivity

Human

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1. Thr32-His266 Accession Number O94907

Conjugate

Application Notes

Immunohistochemistry: 8-25 μg/mL; ELISA Capture: 2-8 μg/mL; ELISA Detection: 0.1-0.4 μg/mL
We recommend the following for sandwich ELISA (Capture - Detection):
DCABY-3999 - DPABY-394

Alternative Names

DKK1; dickkopf WNT signaling pathway inhibitor 1; SK; DKK-1; dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1;

Entrez Gene ID

UniProt ID

Antigen Description

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

Pathway

Cardiac Progenitor Differentiation; Direct p53 effectors; Presenilin action in Notch and Wnt signaling; Regulation of Wnt-mediated beta catenin signaling and target gene transcription; Signal Transduction; Signaling by Wnt; TCF dependent signaling in resp