Anti-DGCR8 monoclonal antibody Made to order

Name: Anti-DGCR8 monoclonal antibody
SKU: DCABH-15164
Rating: 5 (1 reviews)

Rabbit anti-Human DGCR8 monoclonal antibody for WB, ELISA

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

A synthetic peptide of human DGCR8 is used for rabbit immunization.

Conjugate

Unconjugated

Target

Alternative Names

DGCR8; DiGeorge syndrome critical region gene 8; C22orf12, chromosome 22 open reading frame 12; microprocessor complex subunit DGCR8; DGCRK6; Gy1; pasha; DiGeorge syndrome critical region 8; C22orf12;

Entrez Gene ID

54487

UniProt ID

Q8WYQ5

Product Background

Antigen Description

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Pathway

Direct p53 effectors, organism-specific biosystem; Gene Expression, organism-specific biosystem; MicroRNA (miRNA) Biogenesis, organism-specific biosystem; Regulatory RNA pathways, organism-specific biosystem;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

Splicing factors as regulators of miRNA biogenesis - links to human disease

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Authors: Ratnadiwakara, Madara; Mohenska, Monika; Anko, Minna-Liisa

Abstract

MicroRNAs ( miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally by fine-tuning mRNA levels and translation during development and in adult tissues. miRNAs are transcribed as parts of longer precursors that undergo multiple processing steps before the mature miRNAs reach their target mRNAs in the cytoplasm. In addition to Drosha/DGCR8 and Dicer that are the essential components of the miRNA processing pathway, a range of other RNA binding proteins have recently been implicated in miRNA biogenesis. Among these, several well-known splicing factors have emerged as regulators of distinct miRNAs. In this review, we examine the mechanisms by which splicing factors regulate miRNA biogenesis. As both splicing factors and miRNAs play central roles in human disease biology we discuss implications of the links between splicing factors and miRNAs in human disease. (C) 2017 Elsevier Ltd. All rights reserved.

MicroRNA Gene Polymorphisms in Congenital Anomalies of the Kidney and Urinary Tract

CUKUROVA MEDICAL JOURNAL

Authors: Tezol, Ozlem; Delibas, Ali; Ay, Ozlem Izci; Karakas, Umit; Tasdelen, Bahar; Erdal, Mehmet Emin

Abstract

Purpose: Pathogenesis of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) is unknown. A strong genetic contribution is emphasized. In this study we investigated the role of microRNA gene polymorphism in CAKUT. Material and Methods: 147 patients with CAKUT [(Ureteropelvic junction obstruction n: 39, vesicoureteral reflux (VUR) (n: 37), renal parenchymal malformations (n: 43), anomalies of renal embryonic migration (n: 28)] and 51 healthy children were enrolled in the study. RNASEN, DGCR8, XPO5, RAN, DICER1, GEMIN3 gene polymorphisms were studied. Results: When the patient and control group were compared by polymorphisms no statistically significant difference was found. But GEMIN3 mutant allel frequency was significantly higher in VUR group than renal parenchymal malformation group. Conclusions: Mutant alleles of the GEMIN3 gene might be related to VUR pathogenesis within the context of the CAKUT spectrum. But studies with larger number of patients are required to delineate the association between CAKUT pathogenesis and microRNA gene polymorphisms.