Anti-DDX39B monoclonal antibody, clone FQS24255-73

Antibody Isotype

IgG

Clone

FQS24255-73

Species Reactivity

Mouse; Rat; Human

Immunogen

Recombinant fragment within Human UAP56 aa 300 to the C-terminus. The exact sequence is proprietary.Database link: Q13838

Conjugate

Application Notes

Flow Cyt: 1/10; WB: 1/10000 - 1/50000; IHC-P: 1/100 - 1/250; ICC/IF: 1/100 - 1/250.

Alternative Names

DDX39B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B; BAT1, HLA B associated transcript 1; spliceosome RNA helicase DDX39B; D6S81E; U2AF65 associated protein 56; UAP56; spliceosome RNA helicase BAT1; ATP-dependent RNA helicase p47; 56 kDa U2AF65-associated

Entrez Gene ID

UniProt ID

Antigen Description

Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5 end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposis sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5 or 3 overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

Pathway

Exon junction complex (EJC), organism-specific biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem;