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Background
Cy5 is a member of the cyanine dye family, which includes dyes like Cy2, Cy3, Cy5.5, and Cy7. The unique chemical structure of cyanine dyes consists of a partially saturated indole nitrogen heterocyclic nucleus with two aromatic units connected via a polyalkene bridge of varying carbon numbers. Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. These dyes cover a broad spectrum from infrared to ultraviolet wavelengths and are known for increasing the sensitivity range of photographic emulsions. Cyanines typically exhibit green or light blue colors and are chemically unstable. In the context of anti-cyanine antibodies, they can be immobilized and used to bind Cyanine dyes in a linker system. This linker system involves connecting a small molecule reactive group, such as an activity-based probe, to an affinity molecule through an aryl diazo linker.
Figure 1. Prototypical cyanine dye resonance structures. (Source:Alia, J. D. et al., 2020)
Anti-Cy5 Monoclonal Antibody is a type of antibody that specifically recognizes and binds to Cy5. It has been extensively characterized and validated for its ability to detect Cy5 in various experimental techniques, including Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). This antibody enables researchers to specifically target and visualize Cy5-labeled molecules or structures of interest, providing valuable insights into cellular processes, protein interactions, and molecular dynamics. Furthermore, the Anti-Cy5 Monoclonal Antibody is available in different forms to suit different experimental needs. It can be obtained as a non-conjugated form, which allows for customization and conjugation to other molecules or labels. Additionally, it is also available in conjugated forms with molecules such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. These conjugated forms provide convenient options for direct detection and visualization of Cy5-labeled targets without the need for additional secondary antibodies or detection reagents.
Alternative Names
Anti-Cyanine 5 Monoclonal Antibody
References
1. Alia J D, Flack J A. Unspecified verticality of Franck–Condon transitions, absorption and emission spectra of cyanine dyes, and a classically inspired approximation. RSC Aadvances. 2020, 10(70): 43153-43167.
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References
Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time
Here we present a technique called single-molecule high-resolution colocalization (SHREC) of fluorescent dyes that allows the measurement of interfluorophore distances in macromolecules and macromolecular complexes with better than 10-nm resolution. By using two chromatically differing fluorescent molecules as probes, we are able to circumvent the Rayleigh criterion and measure distances much smaller than 250 nm. The probes are imaged separately and localized individually with high precision. The registration between the two imaging channels is measured by using fiduciary markers, and the centers of the two probes are mapped onto the same space. Multiple measurements can be made before the fluorophores photobleach, allowing intramolecular and intermolecular distances to be tracked through time. This technique's lower resolution limit lies at the upper resolution limit of single molecule FRET (smFRET) microscopy. The instrumentation and fluorophores used for SHREC can also be used for smFRET, allowing the two types of measurements to be made interchangeably, covering a wide range of interfluorophore distances. A dual-labeled duplex DNA molecule (30 bp) was used as a 10-nm molecular ruler to confirm the validity of the method. We also used SHREC to study the motion of myosin V. We directly observed myosin V's alternating heads while it walked hand-over-hand along an actin filament.
Chemistry of cyanine dyes-A review
Materials Today: Proceedings
Authors: Gopika G S, Prasad P M H, Lekshmi A G, et al.
Compounds which impart colour is usually referred to as dyes. Based on the difference in the structure they are classified into apocyanines, cyanines, hemicyanines, etc. Owing to the numerous applications in the field of medicine, science and technology, cyanine dyes occupy an indispensable position among dyes and pigments. They are put to use in various ways - as synthetic drugs, photoreceptors, cell growth inhibitors, fluorescent sensors, photorefractive materials etc. Considerable amount of work has been carried out based on the syntheses and applications of cyanine dyes. This review throws light on the structure and classification of cyanine dyes along with its syntheses, aggregation properties and applications.
COA
Publication 
Figure 1. Prototypical cyanine dye resonance structures.
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