General Notes
This product (Catalog No. DPABH-09422) is a rabbit-derived polyclonal antibody raised against human CYP11B1, targeting the amino acid region 134–503 of the protein. It has been affinity purified by Protein A. Steroid 11β-monooxygenase, also known as 11β-hydroxylase or cytochrome P450 11β (CYP11B1), is a mitochondrial enzyme in the cytochrome P450 family that catalyzes the 11β-hydroxylation of steroid precursors, specifically converting 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone in the adrenal cortex. This reaction requires reduced adrenodoxin as an electron donor, molecular oxygen, and protons, following the general form: a steroid + 2 reduced adrenodoxin + O₂ + 2 H⁺ → an 11β-hydroxysteroid + 2 oxidized adrenodoxin + H₂O. Expressed primarily in the zona fasciculata and zona glomerulosa of the adrenal glands, the enzyme is regulated by adrenocorticotropic hormone (ACTH) from the pituitary gland and is essential for glucocorticoid and mineralocorticoid hormone synthesis, which maintain blood glucose levels, respond to stress, suppress inflammation, and regulate electrolyte balance.
DPABH-09422 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:500–1:2,000) applications.
DPABH-09422 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.5% BSA, 0.02% sodium azide. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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