Rabbit Anti-Human CSNK2A1 (a.a. 201-300) Polyclonal antibody

Name: Rabbit Anti-Human CSNK2A1 (a.a. 201-300) Polyclonal antibody
SKU: DPABH-00833
Rating: 5 (1 reviews)

Rabbit Anti-Human CSNK2A1 (a.a. 201-300) Polyclonal antibody for WB

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COA

SDS

Datasheet

Key features and details

Target: CSNK2A1
Host: Rabbit
Application: WB
Species Reactivity: Mouse, Rat, Human, Dog, Cow

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Mouse; Rat; Human; Dog; Cow

Immunogen

KLH conjugated synthetic peptide derived from human CK II alpha: 201-300/391

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-2000

General Notes

This product (Catalog No. DPABH-00833) is a rabbit-derived IgG polyclonal antibody raised against human CSNK2A1, targeting the amino acid region 201–300 of the protein. This antibody exhibits cross-reactivity with CSNK2A1 from mouse, rat, dog, and cow. It has been affinity purified by Protein A. Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
DPABH-00833 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
DPABH-00833 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

STK; Casein kinase II Alpha; CKII alpha; Serine/Threonine Protein Kinase II Alpha; CKⅡalpha; Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1 subunit; Casein kinase II alpha chain; Casein kinase II alpha subunit; Casein kinase II subunit alpha; Casein kinase II alpha 1; CK II alpha; CKIIalpha; CSK21_HUMAN; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CSNK2A1.; CK2α

Entrez Gene ID

1457

UniProt ID

P68400

Citations

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Documents

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References

Molecular characterization of clear cell renal cell carcinoma identifies CSNK2A1, SPP1 and DEFB1 as promising novel prognostic markers

APMIS

Authors: Rabjerg, Maj; Bjerregaard, Henriette; Halekoh, Ulrich; Jensen, Boye L.; Walter, Steen; Marcussen, Niels

Abstract

The prognosis associated with clear cell renal carcinoma (ccRCC) can vary widely and novel molecular prognostic markers are needed to assess prognosis at an earlier stage. Several gene products have been investigated for this purpose, but none of them have been implemented in clinical practice. Here we hypothesized that we, using TaqMan((R)) Array, could identify superior prognostic messenger RNA (mRNA)s in long-term follow-up. Messenger RNA level of 19 candidate genes was investigated in 97 patients with ccRCC. Three genes impacted significantly on prognosis in both univariate and multivariate analysis. In univariate analysis, CSNK2A1 was a strong indicator of a poor overall survival (OS) (HR = 5.01, p < 0.001), disease specific survival (DSS) (HR = 6.21, p = 0.007) and progression free survival (PFS) (HR = 5.93, p = 0.005). High expression of SPP1 was associated to poor PFS (HR = 4.41, p = 0.04). DEFB1 was associated with a better PFS (HR = 0.24, p = 0.006). In multivariate analysis, CSNK2A1 was associated to a worse OS (HR = 3.56, p = 0.008) and PFS (HR = 3.84, p = 0.005), whereas SPP1 was an independent predictor of a worse PFS (HR = 3.46, p = 0.007) and DEFB1 of a better PFS (HR = 0.37, p = 0.027). These results show that with TaqMan((R)) Array we could identify three superior gene products related to prognosis. Further studies are needed to elucidate the pathways and roles of these genes in renal cancer development.

Murine protein kinase CK2 alpha ': cDNA and genomic cloning and chromosomal mapping

GENOMICS

Authors: Xu, X; Rich, ES; Seldin, DC

Abstract

Protein kinase CK2 (casein kinase II) is sl heterotetrameric enzyme implicated in many essential regulatory pathways in cells. We have determined the sequence of the murine CK2 alpha' cDNA that encodes a 350-amino-acid protein that would have 99 and 98% homology with the human and chicken proteins, respectively, and is also highly homologous to murine CK2 alpha. To clarify the sequence of the 5' end of the cDNA and to elucidate the structure and regulation of the gene, we obtained a bacterial artificial chromosome clone that contains the 35-kb CK2 alpha' gene. The gene consists of 12 small exons; the 5' end, including the first exon and intron, is extremely GC rich and contains a CPG island. The putative promoter contains potential binding sites for a variety of transcriptional factors but appears to lack CCAAT- or TATA-like elements. A polymorphic dinucleotide repeat in the fifth intron allowed us to map the CK2 alpha' gene to murine Chromosome 8. (C) 1998 Academic Press.